3D PHASE: PHASE MICROSCOPY AND TOMOGRAPHY – NEW APPROACH TO 3D MEASUREMENTS OF BIOLOGICAL AND TECHNOLOGICAL STRUCTURES
The main objective of the project is to provide such progress in the TIE based methods of an object phase recovery which would enable to design and develop a novel quantitative imaging of phase object instrumentation which bridge a conventional microscope with a digital holographic microscope. This instrumentation will have a simple construction (object beam only) and will provide accurate measurement of entire complex wave. This solution will allow to addresses the next objective: development of tomographic methods and instrumentation for determination of 3D (n(x,y,z)) and 4D (n(x,y,z;t)) refractive index distribution in a wide optical spectrum range. This objective meets the needs of multidisciplinary science for accurate, reliable and simple way of investigations technological and biological samples.
There are three major breakthroughs and goals of the project: development of novel methods and algorithms capable to retrieve integrated and 3D phase distribution from a variety of technological and biological microobjects, design and development of high resolution and single beam phase microscope for wide range of samples, design and development of compact ODT systems capable of 3D and 4D reconstructions.[more]